Vets at London Zoo made a "heart-breaking" decision to put down a 10-week-old Sumatran tiger cub following multiple unexplained seizures.
Following four seizures, each worse than the last, vets euthanised Loki on February 24.
Sharing the news on the ZSL website, chief operating officer Kathryn England said: "Being responsible for an animal’s welfare, means sometimes making decisions that break your heart. But we know we made the right one.
"We had all fallen in love with little Loki and know that many of you had too. We wish we had different news for you but are so comforted by the outpouring of kindness and compassion you’ve shown us already this week."
A post mortem is being carried out with results expected next week.
Loki was born on December 12 to mum Gaysha and dad Asim.
One of an original litter of three, the cub’s two siblings sadly did not survive labour.
Ms England said Loki suffered his first seizure on February 18 and was rushed to the veterinary hospital for medication.
Treatment stopped the first seizure but he needed medication for lethargy which appeared to perk him up.
As plans were made to take him to the Royal Veterinary College, in Camden, the cub had two more seizures.
"Once again there was no obvious cause – in fact he had spent a peaceful Sunday snuggled up with Gaysha," Ms England added.
Loki had emergency MRI, CT scan and multiple blood tests as well as a spinal tap.
"We left no stone unturned. Through our tests we ruled out so many things; a brain tumour, infections, parasites – frustratingly we still had no answers.
" We were considering a presumptive diagnosis of epilepsy – something that can only be confirmed through a process of eliminating all other possibilities."
His condition seemed to improve by the Thursday (February 24) when he enjoyed a calm morning playing outside with his dad.
"Devastatingly, Loki then had another seizure," she said. "Far more serious than those before, the seizure confirmed the vets’ worst fears; Loki’s fits were not going to stop and his health would only decline."
Ms England added: "We will of course continue our efforts to understand Loki’s condition."
The post mortem is being carried out "in the hope that we can help another tiger cub – and the future of this critically endangered species", she said.
Comments: Our rules
We want our comments to be a lively and valuable part of our community - a place where readers can debate and engage with the most important local issues. The ability to comment on our stories is a privilege, not a right, however, and that privilege may be withdrawn if it is abused or misused.
Please report any comments that break our rules.
Read the rules here